Digital PCR for direct quantification of viruses without DNA extraction
نویسندگان
چکیده
منابع مشابه
Digital PCR for direct quantification of viruses without DNA extraction
DNA extraction before amplification is considered an essential step for quantification of viral DNA using real-time PCR (qPCR). However, this can directly affect the final measurements due to variable DNA yields and removal of inhibitors, which leads to increased inter-laboratory variability of qPCR measurements and reduced agreement on viral loads. Digital PCR (dPCR) might be an advantageous m...
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on the use of formamide and reduced incubation temperatures. Here we describe a modification of FoLT PCR which allows direct amplification from solid tissue samples without the need for any DNA purification steps. Two oligonucleotide primer combinations directed against the murine I82 microglobulin gene were used in this study. Using a standard PCR protocol it was not possible to amplify the ap...
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Typically DNA used in PCR assays is usually extracted according to die phenol-chloroform method (1) or by an alternative 'saltingout' rapid purification (2). Moreover partially purified DNA obtained with rapid procedures have been reported to be suitable for PCR amplification (3). We describe here a more simple and efficient method to amplify DNA directly from whole blood samples without any pu...
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Digital polymerase chain reaction (PCR) is a quantitative PCR technique with potential to be more accurate and precise than real-time quantitative PCR without the need for a standard curve. The digital PCR sample/reaction mix is randomly distributed into a large number of partitions, such that some partitions contain no copies of the nucleic acid template and others contain at least one copy. F...
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Dried blood spots collected on Specimen Collection Cards, commonly called “Guthrie cards” (Figure 1), have been used widely as valuable resources for genetic studies, such as, determination of hereditary diseases like phenylketonuria and congenital hypothyroidism. The ease of storage and transportation of these cards with blood spots also lends them to a variety of large field studies, especial...
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ژورنال
عنوان ژورنال: Analytical and Bioanalytical Chemistry
سال: 2015
ISSN: 1618-2642,1618-2650
DOI: 10.1007/s00216-015-9109-0